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奈良県立医科大学先端医学研究支援機構
医学研究支援センター動物実験施設
Anesthesia procedure
Animal experiment performers are required to ethically and safely carry out anesthesia procedures in accordance with laws and regulations in Japan.
Anesthesia (Mouse)
Component
Concentration
Method
Isoflurane
2-5%
Inhaled
Sevoflurane
2-5%
Inhaled
3-component mixed
Medetomidine
Midazolam
Butorphanol
0.3 mg/kg
4.0 mg/kg
5.0 mg/kg
i.p
Propofol
26 mg/kg
i.v
Thiopental (Psy)
30-40 mg/kg
i.v
2-component mixed ①
Ketamine (Nar)
Medetomidine
75 mg/kg
1 mg/kg
i.p
2-component mixed ②
Ketamine (Nar)
Xylazine
80-100 mg/kg
10 mg/kg
i.p
Psy：Psychotropics
Nar：Narcotics
Anesthesia (Rat)
Component
Concentration
Method
Isoflurane
2-5%
Inhaled
Sevoflurane
2.5-5%
Inhaled
3-component mixed
Medetomidine
Midazolam
Butorphanol
0.15 mg/kg
2.0 mg/kg
2.5 mg/kg
i.p
Propofol
10 mg/kg
i.v
Thiopental (Psy)
10-15 mg/kg
i.v
2-component mixed ①
Ketamine (Nar)
75 mg/kg
Medetomidine
0.5 mg/kg
i.p
2-component mixed ②
Ketamine (Nar)
Xylazine
75-100 mg/kg
10 mg/kg
i.p
Psy：Psychotropics
Nar：Narcotics
Unacceptable Anesthesia
Pentobalbital
Diethyl ether
Urethane
Tribromoethanol
Chloral hydrate
Secobarbital
Expired anesthesia
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